Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains
Identifieur interne : 000068 ( France/Analysis ); précédent : 000067; suivant : 000069Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains
Auteurs : L. T. To [France] ; S. Bernard [France] ; E. Bottreau [France]Source :
- Research in Virology [ 0923-2516 ] ; 1992.
English descriptors
- KwdEn :
- Teeft :
- Adult pigs, Antigen, Antigen expression, Attenuated, Attenuated strains, Aynaud, Bohl, Cell culture supernatants, Cell membrane, Cell surface, Coronavirus, Digestive tract, Gastroenteritis, Glycoprotein, Infectious virus particles, Lactogenic immunity, Lantier, Laude, Mutant, Piglet, Plaque assay, Plasma membrane, Structural antigens, Supernatant, Surface antigens, Survivor selection, Tgev, Tgev strains, Transmembrane glycoprotein, Transmissible, Transmissible gastroenteritis, Transmissible gastroenteritis coronavirus, Transmissible gastroenteritis virus, Viral, Viral antigens, Virol, Virulent, Virulent strain, Virulent strains, Virus, Virus strains.
Abstract
Summary: Three strains of the transmissible gastroenteritis virus (TGEV) possessing different degrees of pathogenicity for piglets were examined for their capacity to express M and S glycoproteins on the infected cell surface using a microwell immunoperoxidase test. These two viral glycoproteins were easily detected on the plasma membrane of 0.1 % paraformaldehyde-fixed swine testis (ST) or pig kidney (RP.D) cells which were infected with high-passaged Purdue-115 and low-passaged D-52 strains and a high-passaged attenuated (188-SG) mutant of TGEV. No significant differences were found between attenuated and virulent strains with regard to the viral antigen expression on the membrane of infected cells over a 14-h period.
Trois souches du virus de la gastroentérite transmissible (GET) possédant une pathogénicité différente pour les porcelets ont été examinés, à l'aide d'une technique d'immunopéroxidase en microplaque, pour leur capacité d'expression des glycoprotéines M et S à la surface des cellules infectées. Ces deux glycoprotéines sont facilement détectées sur la membrane plasmique des cellules ST (testicule du porc) et des cellules RP.D (rein de porc) infectées par trois souches différentes de virus de la GET, et fixées à la paraformaldéhyde. Aucune différence d'expression des antigènes viraux sur la membrane des cellules infectées sont observables en fonction des souches virales et des lignées cellulaires utilisées.
Url:
DOI: 10.1016/S0923-2516(06)80112-3
Affiliations:
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These two viral glycoproteins were easily detected on the plasma membrane of 0.1 % paraformaldehyde-fixed swine testis (ST) or pig kidney (RP.D) cells which were infected with high-passaged Purdue-115 and low-passaged D-52 strains and a high-passaged attenuated (188-SG) mutant of TGEV. No significant differences were found between attenuated and virulent strains with regard to the viral antigen expression on the membrane of infected cells over a 14-h period.</div><div type="abstract" xml:lang="fr">Trois souches du virus de la gastroentérite transmissible (GET) possédant une pathogénicité différente pour les porcelets ont été examinés, à l'aide d'une technique d'immunopéroxidase en microplaque, pour leur capacité d'expression des glycoprotéines M et S à la surface des cellules infectées. Ces deux glycoprotéines sont facilement détectées sur la membrane plasmique des cellules ST (testicule du porc) et des cellules RP.D (rein de porc) infectées par trois souches différentes de virus de la GET, et fixées à la paraformaldéhyde. Aucune différence d'expression des antigènes viraux sur la membrane des cellules infectées sont observables en fonction des souches virales et des lignées cellulaires utilisées.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="To, L T" sort="To, L T" uniqKey="To L" first="L. T." last="To">L. T. To</name></noRegion><name sortKey="Bernard, S" sort="Bernard, S" uniqKey="Bernard S" first="S." last="Bernard">S. Bernard</name><name sortKey="Bottreau, E" sort="Bottreau, E" uniqKey="Bottreau E" first="E." last="Bottreau">E. Bottreau</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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